Figure 4.
TSS mapped to the L1 ASP region. (a) The position of TSS present in the DBTSS is shown highlighted on the consensus sequence of L1Hs [4] between positions 347 and 601. TSS with single and multiple entries present in the database are represented by yellow and blue highlight, respectively. The letters above the sequence mark the 3′ end of the oligonucleotide primers used in RT-PCR (see Table 1). (b) Southern blot RT-PCR analysis of the L1-MET transcripts. The lanes are marked according to the primers used in the PCR. Multiple bands on each lane represented the different splice variants of the L1-MET transcript, as confirmed by sequence analysis.