Table 1.

Analysis of variance for the number of infections per replicate in two clones raised under differing maternal environments and two offspring food environments. The maternal environments (see figure 1) tested were fully crossed between good or stressed ‘juvenile’ (from birth to release of first offspring) or ‘adult’ environments and with maternal exposure or not to parasites (parasite). Offspring food levels were tested within maternal clutch, which was set as a random factor. The minimum model was run using glimmix for Proc Mixed in SAS v.8 with binomial errors: Number of infected females on day 26/total number of females in replicate=covariate+clone+maternal ‘juvenile’ environment+maternal ‘adult’ environment+‘juvenile’בadult’ maternal environments+parasite+clone×parasite+offspring food+clone×offspring food+maternal ‘adult’ environment×offspring food+parasite×offspring food+clone×parasite×offspring food, where covariate, is the batch mean for the proportion of infections in the control replicates, on day 26 and offspring food, offspring food level (high or low) when exposed to parasites.

effect	df1,2	F value	p
covariate	1,170	18.64	<0.0001
clone	1,129	0.01	0.91
maternal juvenile environment	1,137	2.04	0.16
maternal adult environment	1,153	26.46	<0.0001
juvenile×adult maternal environments	1,135	18.59	<0.0001
parasite	1,131	1.82	0.18
clone×parasite	1,130	0.07	0.79
offspring food	1,151	14.03	0.0003
clone×offspring food	1,152	0.31	0.58
maternal adult environment×offspring food	1,151	4.16	0.04
parasite×offspring food	1,152	1.68	0.20
clone×parasite×offspring food	1,152	7.88	0.006