Table III.
Karyotypes of parental and hTERT+ cellsa
| Cell culture | PDL | Karyotype (ICSN diagnoses) |
|---|---|---|
| 82-6 mass culture | 38 | 46, XY [10] |
| 82-6 mass culture (hTERT+) | 89 | 46, XY [10] |
| CP-A mass culture | 20 | 47–53, XY, +4, +5, +9, +20, +0–3 mar [cp15] |
| CP-A clone #7 (hTERT+) | 82 | 46–48, XY,i(8)(q10), +20 [cp10] |
| CP-A clone #19 (hTERT+) | 70 | 47–48, XY, +der(1)t(1;6)(q10;?), +20 [8] |
| CP-B mass culture | 15 | 43–45, XY,der(6)t(6;9;12) )(12pter →12p?11.2::6p?11.2 →6q?13::9? →9?),der(9)t(9;14)(q10;q10),−12, der(14)t(6;14)(p12;q?11.2), der(17)t(1;17)(?p31;q10), del(21)(q22)[cp10] |
| CP-B clone #1 (hTERT+) | 99 | 44–46, XY, −4,der(6)t(6;9;12)(12pter →12p?11.2::6p?11.2 →6q?13::9? →9?),der(9)t(9;14)(q10;q10),−12,der(14)t(6;14)(p12;q?11.2), der(17)t(1;17)(?p31;q10),del(21)(q22),+ mar [cp15] |
| CP-B clone #8 (hTERT+) | 91 | 44–46, XY,der(6)t(6;9;12) )(12pter →12p?11.2::6p?11.2 →6q?13::9? →9?), der(9)t(9;14)(q10;q10),−12,der(14)t(6;14)(p12;q?11.2),der(17)t(1;17) (?p31;q10),+18, del(21)(q22)[cp7] |
| CP-C mass culture | 21 | 43–46,XY,der(9)t(9;17)(p10;q10), der(10)t(10;17)(p10;q10), 17, −21,+r,+1–2 mar[cp10] |
| CP-C clone #2 (hTERT+) | 56 | 43–47,XY,der(9)t(9;17)(p10;q10),−17,−21,+r,+1–2 mar[cp8] |
| CP-C clone #11 (hTERT+) | 64 | 42–45,XY,−4,−9,−14,der(9)t(9;17)(p10;q10),−17,−21[cp11] |
| CP-D mass culture | 14 | 39–40,X,-Y,der(1)t(1;22)(p?35;q?12),der(17)t(17;20)(p10;?),−14,−21,−21–22,multiple non-clonal structural (frequently complex) abnormalities, numerical abnormalities and telomeric associations [cp14] |
| CP-D clone #10 (hTERT+) | 86 | 43–45,XY,der(1)t(1;22)(p?35;q?13),del(2)(q10), der(10)t(10;15)(q10;q10), −15,der(17)t(17;20)(p10;?),idic(22)(q13) [cp11] |
| CP-D clone #12 (hTERT+) | 52 | 44–46,XY,der(1)t(1;22)(p?35;q?12),der(3)t(3;10)(q?11;p?26),dup(6) (q?), −10,der(14;15)(q10;q10),der(17)t(17;20)(p?11;?),−19, der(21)t(3;21)(q?12;p12), idic(22)(q13) [cp10] |
a
The karyotype diagnoses represent the contribution of both G-band and SKY analyses. A ‘?’ indicates a complex chromosomal rearrangement, for which exact breakpoints could not be assigned. Numbers within the brackets represent the number of cells containing the listed karyotype. If the karyotype is the composite from a number cells, this is indicated in brackets by the number of cells prefixed with cp. The clonal aberrations that are not present in the parental culture but are found in the corresponding hTERT+ culture are highlighted in bold type.