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. Author manuscript; available in PMC: 2007 Jun 1.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2006 Jun;47(6):2675–2685. doi: 10.1167/iovs.05-1335

Figure 7.

Figure 7

Cell-type differences in tunicamycin effects on phenotype and junctional localization of N-cadherin in early cultures of clone-YH and hTERT-RPE1 cells. (A) Phase contrast microscopy and N-cadherin immunostaining, showing a tunicamycin-induced increase in epithelial phenotype and N-cadherin junctional distribution in clone-YH, and a decrease in hTERT-RPE1. Nuclei are counterstained with DAPI. Scale bars, 100 μm for phase contrast, and 20 μm for immunostaining images. (B) N-cadherin immunoblot showing the partitioning of glycosylated and deglycosylated (after tunicamycin treatment) N-cadherin to the detergent soluble fraction (sol) or to the largely junctional detergent insoluble fraction (insol).