Fig. 8. The HCF-1N subunit can promote G1 phase progression and S phase entry after improper cytokinesis. (A) Immunofluorescence analysis of the long-term BrdU incorporation assay of multinucleated cells is shown for parental (a–c) and HCF-1N1011/SR (d–f) HeLa cells. (a and d) DAPI staining; (b and e) αHCF-1C staining for endogenous HCF-1; (c and f) αBrdU staining. White arrowheads identify HCF-1-depleted binucleated cells. (B) Immunofluorescence analysis of short-term BrdU incorporation in HCF-1 siRNA-treated HCF-1N1011/SR cells. (a and d) DAPI staining; (b and e) αHCF-1C staining for endogenous HCF-1; (c and f) αBrdU staining. Arrowheads identify BrdU-positive, HCF-1-depleted binucleated cells. Scale bar: 5 µm.