Table 1.
Details of primers and methods used for PCR.
| PCR | primer | sequence | [Mg] | anneal temp (°c) | amplicon size (bp) |
|---|---|---|---|---|---|
| IS1081 | forward | 5-CTGCTCTCGACGTTCATCGCCG | 1.5 | 58 | 135 |
| reverse | 5-GGCACGGGTGTCGAAATCACG | ||||
| RD7 flanking | forward 1 | 5-ATCTTGCGGCCCAATGAATC | 2.0 | 58 | 211 |
| forward 2 | 5-TCGGTCAGCAAGACGTTGAAG | 105 | |||
| reverse | 5-ACTTCAGTGCTGGTTCGTGG | ||||
| RD4 flanking | forward 1 | 5-AATGGTTTGGTCATGACGCCTTC | 2.0 | 58 | 176 |
| forward 2 | 5-TGTGAATTCATACAAGCCGTAGTCG | 142 | |||
| reverse | 5-CCCGTAGCGTTACTGAGAAATTGC | ||||
| RD8 flanking | forward | 5-GAGTCTATATAGTGTGCTCATGGGGCTAGC | 2.0 | 64 | 178 |
| reverse | 5-GCTTGCTGGCGATCATTGGTCT | ||||
| RD13 flanking | forward | 5-ATCGCTCGTTCGTCGGCTTC | 2.0 | 60 | 136 |
| reverse | 5-GGCAAGACCGGGCCTTTGAC |