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. Author manuscript; available in PMC: 2006 Sep 5.
Published in final edited form as: Biochemistry. 2006 Apr 4;45(13):4210–4219. doi: 10.1021/bi052382x

Figure 4.

Figure 4

Immunoblotting of eyecup extracts from wild-type and Rdh5−/−Rdh11−/− mice probed with anti-rhodopsin (1D4), anti-RPE65, or anti-LRAT antibodies. The eyecup extracts were prepared from mice by homogenizing with the sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) sample buffer. The proteins (30 μg) were analyzed by 12.5% SDS–PAGE. The eyecup extract from Rdh5−/−Rdh11−/− mice showed no significant difference in the levels of rhodopsin, RPE65, or LRAT compared with wild-type mice.

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