Skip to main content
. Author manuscript; available in PMC: 2006 Sep 5.
Published in final edited form as: Biochemistry. 2006 Apr 4;45(13):4210–4219. doi: 10.1021/bi052382x

Figure 7.

Figure 7

Enzymatic production of 13-cis-retinyl esters by Rdh5−/−Rdh11−/− mice. Mice were dark adapted for 48 h, followed by intravenous injection of 40 nmol of all-trans-retinol in 1 μL of DMSO with a 30 gauge needle under anesthesia. Retinoid analysis was performed both 30 min and 2 days after injection. All experiments were carried out in the dark. A representative chromatogram is shown, and the average data from three mice are indicated with standard deviation above the ester peaks (mean ± SD). Retinoids were extracted from 6-week-old eyes and separated on normal-phase HPLC. The box represents cis-retinyl esters (>90% 13-cis-retinyl esters). Peaks 1, 2, 3/3′, and 4 represent cis-retinyl esters, all-trans-retinyl esters, 11-cis-retinal oximes, and all-trans-retinol, respectively.