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. Author manuscript; available in PMC: 2006 Sep 5.
Published in final edited form as: Biochemistry. 2006 Apr 4;45(13):4210–4219. doi: 10.1021/bi052382x

Figure 9.

Figure 9

Influence of retinoid-binding proteins and Ret-NH2 on the formation of 11-cis-retinol and 13-cis-retinol. The isomerization reaction was performed in 10 mM BTP buffer, pH 7.5, containing 1 mM ATP and 10% BSA. UV-treated bovine RPE microsomes were used as a source of enzyme (150 μg of protein). For inhibition assays, all-trans-Ret-NH2 was used at a concentration of 3 mM. The reaction was initiated by adding all-trans-retinol in DMF and a solution of CRALBP or BSA to the final concentration of 10 mM or 10%, respectively. The reaction mixture was incubated in 37 °C for 1 h.