Table 2.
Retinoid Levels in Genetically Varied Mice Lacking Proteins of the Retinoid Cyclea
| genotype | 13-cis-/11-cis- retinyl esters (pmol/eye) | all-trans- retinyl esters (pmol/eye) | 11-cis-retinal (pmol/eye) | 11-cis-retinol (pmol/eye) | all-trans-retinal (pmol/eye) | all-trans-retinol (pmol/eye) |
|---|---|---|---|---|---|---|
| single knockout | ||||||
| WT | 6.6 ± 0.8 | 52.6 ± 4 | 575 ± 30 | 18 ± 4 | 45 ± 6.5 | 4.6 ± 0.6 |
| opsin−/− | 2.0 ± 0.5 | 15.8 ± 4.6 | 13.7 ± 1.2 | 7.1 ± 1.9 | trace | 4.8 ± 1.3 |
| Rpe65−/− | trace | 1488 ± 65 | none | none | 3 ± 0.1 | 7.4 ± 0.7 |
| Rdh5−/− | 155 ± 40 | 47 ± 9.8 | 508 ± 24 | 9.2 ± 3.5 | 22.3 ± 2.8 | 4.8 ± 1.3 |
| Lrat−/− | trace | trace | trace | trace | trace | 7.9 ± 1.3 |
| Rdh11−/− | 12 ± 1.4 | 67 ± 13.4 | 510 ± 24 | 13.1 ± 1.1 | 17 ± 0.1 | 0.8 ± 1.5 |
| Prrdh−/− | 12 ± 0.8 | 62 ± 4.2 | 514 ± 30 | 20 ± 7 | 40.9 ± 9.4 | 7.9 ± 1.0 |
| double knockout | ||||||
| opsin−/−Rpe65−/− | 34 ± 7.5 | 288 ± 107 | none | trace | trace | 89 ± 3.2 |
| opsin−/−Rdh5−/− | 343 ± 91 | 88.8 ± 29 | 10.1 ± 3.9 | trace | trace | trace |
| Rdh5−/−Rpe65−/− | 140 ± 24 | 744 ± 98 | none | trace | trace | 96 ± 21 |
| Lrat−/−Rpe65−/− | trace | trace | none | trace | trace | 8 ± 2.1.4 |
| Prrdh−/−Rdh5−/− | 162 ± 17 | 39 ± 7.9 | 536 ± 39 | 19.7 ± 2.5 | 9.2 ± 6.5 | 3.5 ± 1.0 |
| Rdh5−/−Rdh11−/− | 186 ± 34 | 104 ± 6 | 480 ± 18 | trace | 29.5 ± 2.0 | trace |
a
Mice were genotyped, and retinoid analysis was performed as described in Experimental Procedures. cis-Retinyl esters were integrated as 13-cis-retinyl esters (>90% cis-retinyl esters).