Table 5.
Plasmids and E. coli strains used in this study.
| Strain or plasmid | Relevant characteristicsa | Source or reference |
| Strains | ||
| DH5α | supE44 ΔlacU169 (ϕ80 lacZ ΔM15)hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Lab collection |
| ATCC 43895 | stx1 and stx2; other designation EDL 933W | Lab collection |
| SY327 λpir | Δ(lac pro) argE(Am) recA56 nalA Rif(λpir); π protein for R6K γ ori | [57] |
| SM10 λpir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu, λpir, oriT of RP4; Kmr | [57] |
| FRIK47001Pb | ATCC 43895, hns::nptI | This study |
| Plasmids | ||
| pBR322 | Apr and Tcr | Lab collection |
| pUC4K | pUC4 with nptI; Apr, Kmr | Pharmacia, [44] |
| pCVD442 | R6K γ ori, sacB, oriT of RP4; Apr | J. Kaper, [45] |
| pSC9916 | pBR322 containing Apr, Tcs | [47] |
| pSC0061 | pSC9916 containing hns; Tcs, Apr | This study |
| pSC0062 | pSC0061, hns::nptI | This study |
| pSC0063 | pCVD442 containing hns::nptI | This study |
a Apr, ampicillin resistant; Aps, ampicillin sensitive; Kmr, kanamycin resistant; Tcr, tetracycline resistant; Tcs, tetracycline sensitive. Antibiotics were added to media when appropriate at the following concentrations: ampicillin 100 μg/ml, kanamycin 50 μg/ml, tetracycline 10 μg/ml.
b FRIK47001P hns::nptI in the parallel orientation.