Figure 2.

Transient targeting of motor axons and disorganization of nerve terminals in the diaphragm. (A–C) Wild type and (D–F) mutant. At E14, axons terminated at the central endplate band (arrow in B) in the controls (A and B; A, low power; B, higher power). In the mutant embryos, axonal terminals were also located at the central band (arrow in E) of the muscle (D, low-power view; E, high-power view). However, the preterminal nerves (arrowhead in E) and terminals (arrow in E) were disorganized in the mutants, as compared with those in the wild type (B). At E15.5, nerve terminals degenerated in the mutant (F) as compared with the wild-type embryo (C). [Bars = 200 μm (A and B) and 100 μm (C–F).]