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. 2006 Sep 7;116(10):2767–2776. doi: 10.1172/JCI26893

Figure 2. Levels of SR-BI expression.

Figure 2

(A) For each SR-BI genotype, total protein extracts were prepared from the indicated tissues and loaded onto SDS-PAGE, followed by immunoblotting with an anti–SR-BI antibody (or with an anti–SR-BII antibody for liver extracts) directed against the C-terminal domain of the protein. WAT, white adipose tissue; BMD-M, BM-derived macrophages. (B) Relative SR-BI mRNA levels. Total RNAs extracted from tissues were pooled (4–8 mice per group), reverse-transcribed, and subjected to real-time PCR quantification. Values (mean ± SD) represent the amount of SR-BI mRNA relative to that measured in control mice, which was set arbitrarily to 1. Data for BM-derived macrophages were generated from 1 representative RNA preparation for each SR-BI genotype. *P < 0.05 compared with controls.