Figure 3. Impact of changes in SR-BI expression on plasma lipoprotein cholesterol profiles.
(A) Fasting plasma samples (pooled plasma from 6–10 mice) from 2-month-old female mice fed a chow diet were fractionated by size-exclusion chromatography. TC was measured in each fraction to determine lipoprotein cholesterol profiles. Approximate elution volumes for particles in the size ranges of VLDL, LDL, and HDL are indicated. (B) Electrophoretic mobilities of plasma lipoproteins. Plasma samples (10 μl) were subjected to agarose gel electrophoresis and lipoproteins revealed by Sudan black staining. α, pre-β, and β positions corresponding to migrations of HDL, VLDL, and LDL particles, respectively, are indicated.