Figure 8. Keratinocytes from a subset of psoriatic patients express higher CXCL8 mRNA levels in response to TPA.

(A) Real-time PCR analysis of CXCL8 mRNA expression in keratinocytes cultured from lesional skin biopsy samples from a psoriatic patient or skin of a healthy control treated with increasing doses of TPA. (B) CXCL8 mRNA expression in patient and control keratinocyte cultures shown in Figure 7. Cultures were stimulated for 1 hour with TPA (5 ng/ml) with or without incubation with 5 μM Gö 6976 for the previous 30 minutes (B). “Normal” is representative of cultured keratinocytes from 3 healthy controls. Bars represent mean value of triplicate determinations from a single patient. Results are expressed as fold increase in expression compared with that in untreated normal keratinocytes.