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. 2006 Aug 24;25(17):3986–3997. doi: 10.1038/sj.emboj.7601291

Figure 2.

Figure 2

The expression of the known DSB repair genes is not significantly affected by SWI/SNF inactivation. (A) The fold changes of the expression of thus far known 24 DBS repair genes by SWI/SNF inactivation are summarized (these are all below the cutoff value). The data sources for the fold change of each gene are indicated in the last column. Note that the majority of the genes (17 genes) were changed by less than 1.2-fold by SWI/SNF inactivation, and that Rad51 and Rad51D were decreased by only 1.3-fold and the remaining five genes were rather increased by SWI/SNF inactivation. (B) (Top) The effects of SWI/SNF inactivation on the expression of the DSB repair genes that were not included in the mouse 11K gene chip. RT–PCR was performed using total RNA isolated from B05-1(+tet) and B05-1(−tet) cells. The mRNA expression of GAPDH was analyzed as internal control. The predicted sizes of the PCR products are as follow: RAD54B, 614 bp; EME1, 681 bp; LIG4, 641 bp; Artemis, 655 bp; GAPDH, 361 bp. The first lane (M) is 100-bp standard size marker (the most bottom band is 400 bp). (Bottom) The expression of flag-BRG-1 and α-tubulin (internal control) from the cells used in the top panel was analyzed by immunoblottings.