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. 2006 Aug 24;25(17):4050–4060. doi: 10.1038/sj.emboj.7601288

Figure 2.

Figure 2

Cholesterol promotes AChR cluster assembly at original and ectopic NMJs in paralyzed DeSyn muscles. Low- (A) and high-magnification (B) views of presynaptic nerves (mGFP) and postsynaptic AChR clusters (RITC-α-BT) in lateral gastrocnemius muscles treated with Botulinum toxin A (BotA). The chronic BotA treatment elicited a massive nerve sprouting response in this DeSyn muscle; cholesterol promoted AChR cluster assembly, and inhibited nerve sprouting. Note pretzel-shaped AChR clusters (arrows, right) induced by sprouts (arrows, left) in the presence of exogenous cholesterol. Bars: 200 (A) and 40 μm (B).