Figure 3.

Cholesterol stabilizes AChR clusters in src^−/−;fyn^−/− myotubes. (A) src^−/−;fyn^−/− myotubes were not treated or stimulated overnight with 1 nM agrin to induce AChR clusters (top row). Agrin was withdrawn, cells were washed and incubated for 5 h in agrin-free medium lacking (bottom left) or containing (bottom right) 75 μM cholesterol. Myotubes were stained with rhodamine-α-BT to visualize AChR clusters. (B) For cluster quantification, visual fields covering about three times the area of a panel shown in (A) were taken, and only compact clusters with a mimimum size of 5 μm were counted.