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Effect of malonyl-CoA and short-chain acyl-CoA thioesters on FapR-mediated repression. In vitro transcription was performed with a PfapR promoter fragment (6.4 nM) as the template in the presence of FapR (120 nM) and different concentrations of malonyl-CoA (upper panel) or malonyl-CoA analogs (lower panel).
