Figure 4.
Spindle checkpoint signaling fails following depletion of Nnf1R. (A, C) Cumulative frequency plots of anaphase onset times in siRNA-treated cells, as indicated, with NEB=T0, as determined from live-cell movies. (B) Percentage mitotic arrest in siLaminA-, siNnf1R-1-, siNnf1R-3 or siMad2-transfected cells treated with nocodazole for 16 h. (D) siLaminA-, siNnf1R-1- or siNnf1R-3-treated cells stained with CENP-A (KT marker), Mad2, Mad1, Bub1 or BubR1 antisera following treatment with nocodazole and MG132. Scale bar=10 μm. (E) Quantification of immunofluorescence signals as described in Figure 2A.