Figure 7.
Spindle checkpoint failure in Mcm21R-depleted cells. (A) Percentage mitotic arrest in siLaminA-, siMcm21R-1- or siMad2-transfected cells treated with nocodazole for 16 h. (B) Cumulative frequency plots of anaphase onset times in siRNA-treated cells, as indicated, with NBD=T0, as determined from live-cell movies. (C) Quantification, as described in Figure 2A, of Bub1, BubR1, Mad1 and Mad2 proteins levels on KTs following siLaminA or siMcm21R-1 treatment. (D) Representative immunofluorescence images of cells from (C) stained with CREST, Mad2, Mad1, Bub1 or BubR1 antisera following treatment with nocodazole and MG132. Scale bar=10 μm.