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. 2006 Aug 24;25(17):4033–4049. doi: 10.1038/sj.emboj.7601293

Figure 9.

Figure 9

Dynamics of spindle assembly in Mcm21R-depleted cells. Successive frames every 3 min from live-cell movies of H2B-GFP/α-tubulin-mRED-expressing HeLa cells transfected with siLaminA (A) or siMcm21R-1 (B). Shown are the composite images for H2B-GFP (green) and α-tubulin-mRED (red) or the single channel for α-tubulin-mRED (lower panels). Arrows indicate anaphase movement in siMcm21R-1-treated cells. Note chromosome decondensation in both cells 18 min after anaphase onset. Scale bar=10 μm. (C) Quantification of bipolar spindle formation errors at T=12 min after NBD.