Table 1.
Target 1) | Primer pair 2) | Ampl. (bp) |
intron 2–15 | p1255: ggaattctggagctgcttgggaacca m3652: cgtctagagccggacactcagccttca |
2409 |
intron 2 | p1252: tgtttctggagctgcttgggaacca m1725: tcatcagccagtgcaggaacttg |
474 |
intron 2–3 | p1509: tggggctccaggcacaacgaa m1955: cttggggatgaagcggagtct |
447 |
intron 4 | p1870: tgcgggagctgtcggaagcagag m2170: cgcacacgcagcacgaaggt |
301 |
intron 5–9 | p2182: cgccgcctgagctgtactttgtc m2681: gctctagatccaccaaacgcaggagca |
507 |
intron 10–13 | p2660: gctgctcctgcgtttggtggatgat m3164: ggggttcttccaaacttgctgatg |
505 |
intron 14–15 | p3194: ggcctccctctgctactccatcct m3652: cgtctagagccggacactcagccttca |
466 |
intron 14–15 | p3194: ggcctccctctgctactccatcct m3925: gggcacacctttggtcactcc |
732 |
1) Positions of indicated introns are included in PCR product. 2) Primer sequences are from 5' to 3'. p, forward primer; m, reverse primer.