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Caspase-1 activity measured as fluorescence by using acetyl-Tyr-Val-Ala-Asp-amino-4-methyl coumarin, present in lysates prepared from cells incubated for 2 h in buffer containing air and 5% CO2 without or with 100 μM SIITU or the same buffer plus 110 nM CO without or with 100 μM SIITU. Values are means ± SEM; numbers in parentheses indicate the number of trials. *, P < 0.05 (ANOVA). RFU, relative fluorescence units.
