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. 2003 May 15;31(10):2534–2543. doi: 10.1093/nar/gkg358

Figure 1.

Figure 1

Phenotypes, experimental procedures for and results of PCR-select cDNA subtraction. (A) Seeds of wild-type upland cotton Xuzhou 142 (left) and fl (fuzzless-lintless) mutant (right). (B) Strategy for isolating and identifying cotton fiber development-related genes via PCR-select cDNA subtraction and differential screening. (C) Products of PCR-select cDNA subtraction. M, Φ174/HaeIII digested molecular marker; UD, unsubtracted driver cDNA; UT, unsubtracted tester cDNA; S1, PCR products obtained after one round of subtraction; S2, PCR products obtained after two rounds of subtraction.