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. 2003 May 15;31(10):2534–2543. doi: 10.1093/nar/gkg358

Figure 4.

Figure 4

RT–PCR analysis of cotton fiber cDNAs. Total RNA (5 µg) isolated from fl mutant ovules and from fiber cells at different developmental stages was used to synthesize first-strand cDNA for RT–PCR using gene-specific primers. A cotton ubiquitin cDNA was used to normalize the amount of templates added in PCR reactions. M, fl mutant ovule; UF, upland cotton fiber. Days post anthesis (d.p.a., days 0–20) are shown at the top. Capitalized gene names indicate previously reported fiber genes that were not recovered from our subtractive library. These genes did not show significant differences in expression between 10 d.p.a. fiber and fl mutant ovules. KCBP, kinesin-like calmodulin binding protein; VLCFA, very-long chain fatty acid; CAP, adenyl cyclase associated protein; RGP, reversibly glycosylated polypeptide.