Figure 2.
msq encodes zebrafish ILK and is highly conserved between different species. (A) Integrated genetic and physical map of the zebrafish msq region. The msq mutation interval is flanked by the microsatellite markers z7504 and z7028. A bacterial artificial chromosome (BAC) clone CH211-287A12 and sequence contigs ctg1169.1 and NA 3646.1 (Sanger assembly Zv2) cover the msq interval and encode three ORFs, highly homologous to human Sphingomyelinase, ILK, and an unknown protein (Riken 2210415M20). The genomic structure of zebrafish ilk (zilk) is displayed at the bottom of the figure. The msq missense mutation (T ® C) in the eighth exon of zilk is indicated. (B) The msq missense mutation (T ® C) at cDNA position 923 translates into an amino acid exchange from leucine (L) to proline (P) (CTA ® CCA). The mutated base is marked by an arrow. (C) Amino acid sequence alignment of zebrafish (zILK), human (hILK), mouse (mILK), and D. melanogaster (dILK) ILK demonstrates high amino acid identity cross-species. Black boxes indicate amino acid identity. Functional domains, such as ankyrin domains (ANK), a PH domain, and the C-terminal ILK kinase domain are indicated with bars above the alignment. The msq mutation ILKL308P resides within the kinase domain of ILK.