Figure 2.

Immobilized Emi1 can bind to the APC/C^Cdh1 in an “APC/C capture” assay. (A) Recombinant Emi1 protein immobilized on Sepharose beads (see Materials and Methods) was incubated with HeLa cell extracts, and precipitates were analyzed for APC/C binding by immunoblot analysis. The approximate molecular weight at which the indicated subunit migrates is indicated in parentheses. (B) Emi1 binds to the APC/C in the absence of Cdh1. Emi1 beads were incubated with extracts made from HeLa cells transfected with siRNA targeting Cdh1 or APC3 for 48 h.