Figure 2.

Microtubules persisted at cell tips when Peg1 or dynein heavy-chain functions were compromised. GFP α-tubulin was expressed by the atb2.nmt81GFP allele in the indicated strains at 36°C. Each panel shows, from the top, a 10-sec-interval series of maximal projections of the indicated strains. The arrows highlight microtubule tips that will pause at the cell end for the period indicated by the boxes around the frames. Thus, the microtubule in the example of a wild-type cell pauses at the right tip for 30 sec, while in peg1.1, the microtubule resides at the cell tip for 100 sec. Numbers in the corner of panels indicate relative timing in seconds.