Figure 5.

Peg1 was not a polarity determinant and was not required for the recruitment of Tip1 to microtubules or cell tips. (A) Peg1 was not required to maintain linear growth. Indicated strains were grown at 36°C for 5 h in EMM2 medium before being stained with calcofluor. Shifting nitrogen-starved cells from 25°C to 36°C and then following them as they recovered from the starvation also failed to reveal a morphology defect when Peg1 was compromised (data not shown). (B) tip1.4GFP cells were briefly resuspended in TRITC-conjugated lectin before being washed twice in filter-sterilized minimal medium and imaged using spinning disk microscopy as described in the legend for Figure 3. Cells were either viewed in isolation (left panel) or alongside the indicated double/triple mutants that had not been stained with TRITC lectin.