Figure 5.

Insm1 expression in the developing intestine. Immunohistological analysis of the developing intestine of Insm1^lacZ/+ mice at E15.5 (A,B) and E18.5 (C–F). (A) β-Galactosidase (red) was observed in the intestinal epithelium (arrow) and the enteric nervous system (arrowhead) of Insm1^lacZ/+ mice. (B) β-Galactosidase (red) is coexpressed with NeuroD1 (green) in entero-endocrine cells located in the intestinal epithelium (arrows), but not in the enteric nervous system (arrow-head); coexpressing cells appear yellow, an overlap of the red and green fluorescence. In the intestinal epithelium, all cells that contained chromogranin A (CA, green) (C) or synaptophysin (Syp, green) (D) coexpressed β-galactosidase. In contrast, Mucin2 (Muc2, green) (E) or lysozyme (green) (F) proteins were not observed in the β-galactosidase⁺ (red) cells. Sections were counterstained with TOTO-3 (blue). Bars: A, 50 μm; C, 20 μm.