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. 2003 May 15;31(10):2694–2702. doi: 10.1093/nar/gkg364

Figure 1.

Figure 1

Inhibition of HIV-1 IN-mediated 3′ processing by Vpr and its subdomains. (A) Dose–response effect of full-length Vpr on 3′ processing of a 21mer ODN substrate. The 5′-end-labeled U5 substrate (10 nM) was incubated for 1 h at 37°C with 65 nM IN. Lanes 1–6, 50, 100, 200, 400 and 800 nM, and 1.6 µM Vpr, respectively; lane 7, no Vpr; lane 8, negative control in the absence of IN. The 21mer DNA substrate, 19mer 3′ processed product and the strand transfer products are indicated on the left. (B) Quantification of experimental data obtained in the presence of full-length Vpr (filled circles), (1–51) (open squares) and (52–96) (open diamonds) peptides of Vpr. Data from two independent experiments were averaged.