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. 2003 May 15;31(10):2694–2702. doi: 10.1093/nar/gkg364

Figure 4.

Figure 4

Effects of the overlapping C-terminal peptides of Vpr on mini-viral DNA strand transfer activity. Reactions were performed by incubation for 1 h at 37°C of 2 pmol IN, 10 ng of U3U5 substrate and 40 ng of pSP70 target in the presence of increasing concentrations of Vpr peptides. Concentrations of 0.8, 1.6, 3.2 µM of (52–96) were used in lanes 3–5, of (52–70) in lanes 6–8, of (70–96) in lanes 9–11 and of (60–80) in lanes 12–14, without Vpr peptides in lane 2 or without IN in lane 1. Components were pre-incubated together for 10 min on ice before incubation at 37°C. (A) Phosphorimage of heterologous strand transfer; bands located above the main products correspond to multiple integration events. (B) Phosphorimage of homologous strand transfer products.