Table 4. Incorporation performance of various modified 2′-deoxyribonucleoside-5′-triphosphates.

Reporter groupb	Linker length	dUTP	dATP	dGTP	dCTP
None	None	18a	>18c	18	18
Biotin	12	>18c	>18c	>18c	>18c
RhG	12	14	8d	17–18	11
Cy5	8	10–11	4	n.a.e	5
Evo30	8	14	13d	8	4
GB3	11	13	13d	13d	5
Atto655	8	4d	16	n.a.	8

^aNumber of documented individual incorporation steps out of a possible 18 in the homopolymeric DNA templates, as described in the DNA polymerase assay in Tasara et al. (accompanying manuscript).

^bRhG, Rhodamine Green; Cy5, cyanine 5; Evo30, Evoblue 30; GB3, Gnothis blue 3.

^cTemplate slippage or terminal transferase activity of Vent^R exo^– DNA polymerase observed.

^dLonger incorporation observed, but bands could not be resolved.

^en.a., not available.