Table 2. Hybridization data^a.

ODN	ODN / RNA-22
	0.01 M NaCl Tm (°C)	–ΔG°37 kcal/mol	Δ Tm1b (°C)	ΔTm1/unitc (°C)	Δ Tm2d (°C)	1 M NaCl Tm (°C)	ΔTm3e (°C)
14	33.8 ± 0.4	11.5				56.0	+22.2
15-II	36.6 ± 0.3	12.7	+2.8	+2.8		–
16-II	34.8 ± 0.4	12.5	+1.0	+1.0		–
17-II	37.5 ± 0.1	12.4	+3.7	+3.7		–
18-II	37.9 ± 0.4	12.0	+4.1	+2.1		57.1	+19.2
19-II	43.0 ± 0.1	14.7	+9.2	+3.1		59.3	+16.3
20-II	44.4 ± 0.3	14.6	+10.6	+2.7		58.2	+13.8
21-II	47.2 ± 0.2	14.9	+13.4	+2.7		58.8	+11.6
21-V	30.4		–3.4			49.5	+19.1
15-III	36.3 ± 0.3	12.9	+2.5	+2.5	–0.3	–
16-III	34.2 ± 0.0	11.2	+0.4	+0.4	–0.6	–
17-III	37.2 ± 0.2	12.2	+3.4	+3.4	–0.3	–
18-III	38.1 ± 0.0	12.4	+4.3	+2.2	+0.2	58.1	+20.0
19-III	45.5 ± 0.1	14.5	+11.7	+3.9	+2.5	61.2	+15.7
20-III	47.0 ± 0.0	15.4	+13.2	+3.3	+2.6	60.9	+13.9
21-III	49.0 ± 0.4	16.5	+15.2	+3.0	+1.8	60.9	+11.9
21-VI	30.6		–3.2			50.9	+20.3

^aExperimental conditions are described in Materials and Methods.

^bΔT_m¹ = T_m (each ODN) – T_m (control ODN 14).

^cΔT_m¹/unit = T_m¹ (each ODN)/the number of modification.

^dΔT_m² = T_m (each ODN containing D) – T_m (each ODN containing the same number of H).

^eΔT_m³ = T_m (1 M NaCl) – T_m (0.01 M NaCl).