Table 4. Kinetic parameters of the hydrolysis of RNA-28 in the duplex by E.coli RNase H.
| ODN | Km (µM) | Vmax (µM/min) | kcat (1/s) | kcat/Km (1/s/µM) |
|---|---|---|---|---|
| ODN 24-IV | 3.9 | 0.30 | 5.0 | 1.3 |
| ODN 24-III | 9.6 | 0.011 | 0.19 | 0.02 |
| ODN 24-VI | 10.0 | 0.17 | 2.8 | 0.28 |
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