Fig. 5. B-p-GFP and B-p-GFP-C display distinct activities during T-cell differentiation. (A and D) The CD4/CD8 profiles in the adult thymus of B-p-GFP and B-p-GFP-C transgenic lines were revealed using antibodies to CD4 and CD8 conjugated to Cy-Chrome and R-PE, respectively. Each subpopulation was analyzed for GFP expression relative to its counterpart in a non-transgenic control. Histograms from control and transgenic populations are shown, and the percentage of the population falling within the positive gate is indicated. (B and C) Expression of B-p-GFP (B) and B-p-GFP-C (C) in the double-negative thymic precursors in the adult thymus. After gating out Lin⁺(TCRβ⁺/CD4⁺/CD8⁺/Mac1⁺/Gr1⁺/Ter119⁺/B220⁺/TCRγδ⁺/NK1.1⁺) cells, thymocytes were subdivided further into DN1–DN4 using antibodies to CD44 (APC) and CD25 (R-PE) antigens. The DN1–DN4 subpopulations were analyzed further for GFP expression in transgenic and non-transgenic controls. Histograms for GFP expression are shown, and the percentage of the population falling within the positive gate is indicated.