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Fig. 6. Effect of pH on the 3′–5′ exonuclease and the pyrophosphorylase activities. (A and B) Autoradiograms show the release of terminal nucleotide from TEC-21U by the ED/AA mutant and wild-type RNAPs. (C) Effect of pH on the initial rate of terminal UMP release by the wild-type and mutant RNAPs at 10 mM MgCl2.
