. 2003 May 1;22(9):1990–2003. doi: 10.1093/emboj/cdg197

Table IV. Parameter values for the three-state allosteric kinetic mechanism for the α7/5HT₃ receptor and mutants with five equivalent ACh binding sites per molecule.

α7/5HT₃ receptor parameters
State parameters	B state	A state	D state
ACh on rates (M^–1 s^–1)	^Bk_on = 10⁷	^Ak_on = 3.33 × 10⁶	^Dk_on = 10⁶
ACh off rates (s^–1)	^Bk_off = 880	^Ak_off = 7.5	^Dk_off = 1.6
Equilibrium constants (µM)	^BK_D = 88	^AK_D = 2.25	^DK_D = 1.6
Interconversion parameters	B↔A	A↔D
Transition state positional parameters	^BAp = 0.75	^ADp = 0.99
Interconversion rates (s^–1)	^BAk₅ = 22.8	^ADk₅ = 2.54
	^ABk₅ = 0.107	^DAk₅ = 1.10
	^BAk₀ = 0.233	^ADk₀ = 2.5
	^ABk₀ = 10⁵	^DAk₀ = 6.0
Allosteric constants	^BAL₀ = 4.3 × 10⁵	^ADL₀ = 2.4
	^BAL₅ = 4.7 × 10^–3	^ADL₅ = 0.43
G152K^a, P193I or G152K/P193I mutant parameters
State parameters	B state	A state	D state
ACh on rates (M^–1 s^–1)	^Bk_on = 10⁷	^Ak_on = 3.33 × 10⁶	^Dk_on = 10⁶
ACh off rates (s^–1)	^Bk_off = 1173.33	^Ak_off = 6.66	^Dk_off = 1.42
Equilibrium constants (µM)	^BK_D = 117	^AK_D = 2.0	^DK_D = 1.42
Interconversion parameters	B↔A	A↔D
Transition state positional parameters	^BAp = 0.85	^ADp = 0.99
Interconversion rates (s^–1)	^BAk₅ = 63.5	^ADk₅ = 4.07
	^ABk₅ = 3.06 × 10^–6	^DAk₅ = 0.92
	^BAk₀ = 3.0	^ADk₀ = 4.0
	^ABk₀ = 100	^DAk₀ = 5.0
Allosteric constants	^BAL₀ = 33	^ADL₀ = 1.30
	^BAL₅ = 4.82 × 10^–8	^ADL₅ = 0.226

^aFor the G152K mutant, ^BK_D = 33.2 µM, ^AK_D = 1.2 µM and ^DK_D = 0.2 µM.

Table IV. Parameter values for the three-state allosteric kinetic mechanism for the α7/5HT3 receptor and mutants with five equivalent ACh binding sites per molecule.