Figure 7.

Assembly of PSII Is Altered in slr1471-gfp.

Wild-type, slr1471-gfp, and slr1471-Km^R cells (2 × 10⁶) were pulse- and pulse/chase-labeled with [³⁵S]Met in vivo as described for Figure 5. Total membranes were separated by two-dimensional BN/SDS-PAGE. Complexes containing radiolabeled proteins in the SDS-PAGE size range from 25 to 50 kD are presented in a molecular mass window as indicated in kilodaltons for BN-PAGE. Frames (dotted lines) highlight the positions of PSII assembly complexes [RC and RC*, reaction center complexes of PSII including D1 and D2; RC47, reaction center complex of PSII including CP47; RCC(1) and RCC(2), monomeric and dimeric reaction center core complexes of PSII; PSII_sc, PSII supercomplexes; “free protein,” unassembled protein solubilized from the membrane phase]. Radiolabeled proteins CP47, CP43, D2, pD1, intermediate D1 (iD1), and mature D1 (D1) are indicated. Asterisks mark areas of missing reaction center complexes in slr1471-gfp.