Table 2.

Primers Used for Plasmid Construction, PCR, Quantitative RT-PCR, and RT-PCR Analyses

Primer	Sequence	Gene/Plasmid
Promoter construction
Amy8F	5′-GCCCGGGTGCGTGATCGGTGATCG-3′	pAmy8SRC/GARC
Amy8R	5′-GCGATATCAACAATCAATGATGTTGC-3′	pAmy8SRC/GARC
2TAF	5′-CCTTATCCATATCCATTATCCGTGAATTGCAACAGC-3′	pαAmy8SRC/GARC(2TA)
31KpF	5′-TAACCACCTTTCGAACTGTTGCTTATCCGTG-3′	pαAmy8SRC/GARC(mTA)
34SacF	5′-CCGTTGGAGAAAGCTTTATCCATGTTGC-3′	pαAmy8SRC/GARC(mGARE)
8GARE	5′-CCTACGTGGCCATAAATAACCACCTTATCCATATCCA-3′	pαAmy3SRC(+Amy8GARE)
Quantitative RT-PCR
3RT25A	5′-GTAGGCAGGCTCTCTAGCCTCTAGG-3′	αAmy3
3RTR	5′-AACCTGACATTATATATTGCACC-3′	αAmy3
8RT1	5′-CTCAGGGTTCCTGCCGGTAGAAAGCA-3′	αAmy8
8RTB	5′-CGAAACGAACAGTAGCTAG-3′	αAmy8
GARTF	5′-CAGACGCTAAAGCAGATTC-3′	MYBGA
GARTR	5′-GGCTTATCTCCATGCAC-3′	MYBGA
18SF	5′-CCTATCAACTTTCGATGGTAGGATA-3′	18S rRNA
18SR	5′-CGTTAAGGGATTTAGATTGTACTCATT-3′	18S rRNA
RT-PCR
7RT1	5′-TGAGCGCACGATGACGAGACTCTCA-3′	αAmy7
7RT2	5′-AATTGCATCCGTAATTCGGA-3′	αAmy7
S1RTF	5′-ATGGACGGACATGAGC-3′	MYBS1
S1RTR	5′-GCTTTCACCGGGTGTA-3′	MYBS1
ART1	5′-CTGATGGACAGGTTATCACC-3′	Actin1
ART3	5′-CAGGTAGCAATAGGTATTACAG-3′	Actin1
Genotyping
Myb12-5′	5′-TCAGCTCTCCAAAGTTTCCC-3′	MYBGA
Myb11-3′	5′-CAGGTTCATATTTAGGCCCC-3′	MYBGA
LTR4A	5′-ACTGTATAGTTGGCCCATGTCCAG-3′	Tos17
GAMYB5′	5′-CGCGCCATGTATCGGGTGAAG-3′	MYBGA cDNA
GAMYB3′	5′-CCGTCATTTGAATTCTGACAT-3′	MYBGA cDNA