Table 1.
Fiber-FISH Measurements of the CentO Array in Cen3
| Fibers | A0037J17 + 0047D08 (μm)a | Gap 1 (kb)b | Gap 1 (μm)b | CentO (kb) | CentO (μm) | Gap 2 (kb)b | Gap 2 (μm)b | a0053G10 (μm)a |
|---|---|---|---|---|---|---|---|---|
| 1 | 106.9 | 61.8 | 23.5 | 446.9 | 169.7 | 36.2 | 13.7 | 48.1 |
| 2 | 83.5 | 48.5 | 15.2 | 420.1 | 132.0 | 43.7 | 13.7 | 44.7 |
| 3 | 69.0 | 41.4 | 11.3 | 446.1 | 121.5 | 26.9 | 7.3 | 42.1 |
| 4 | 79.5 | 40.5 | 12.2 | 388.8 | 117.2 | 37.9 | 11.4 | 43.5 |
| 5 | 77.1 | 40.1 | 11.5 | 455.2 | 130.1 | 30.4 | 8.7 | 39.5 |
| 6 | 78.8 | 23.7 | 6.8 | 448.9 | 127.9 | 27.1 | 7.7 | 37.4 |
| 7 | 81.3 | 50.3 | 15.6 | 437.6 | 136.0 | 27.4 | 8.5 | 45.5 |
| 8 | 81.4 | 29.8 | 9.2 | 369.2 | 114.4 | 43.9 | 13.6 | 45.0 |
| 9 | 79.2 | 54.7 | 15.5 | 450.0 | 127.2 | 54.2 | 15.3 | 36.1 |
| 10 | 68.4 | 35.0 | 8.8 | 547.3 | 137.4 | 31.3 | 7.9 | 34.0 |
| Average | 80.5 | 42.6 | 13.0 | 441.0 | 131.3 | 35.9 | 10.8 | 41.6 |
| sd | 10.6 | 11.6 | 4.8 | 47.2 | 15.4 | 9.1 | 3.1 | 4.6 |
a
BACs a0037J17 (150 kb; AC135226) and b0047D08(141 kb; AC137925) overlap 28 kb. Therefore, excluding the overlap, these two BACs generate 263 kb of fiber-FISH signals. BAC a0053G10 is 145 kb (AC091233). As an example, the kb/μm conversion rate for fiber 1 is calculated as (263 + 145)/(106.9 + 48.1) = 2.632.
b
Gap 1 refers to the gap between the signal from BACs a0037J17 and b0047D08 and the signal from CentO; gap 2 refers to the gap between the signal from CentO and the signal from BAC a0053G10.