Figure 4.

Clamp-Like Structures Participate in Nuclear Distribution.

(A) to (E) Dikaryotic hyphae of crosses of strains UMS73 (a1b1) and UMS74 (a2b2).

(F) to (H) Hyphae of the haploid solopathogenic strain UMS71 (a1mfa2bE1/bW2); all strains express a nuclear-localized NLS-3xeGFP; nuclei are visualized by fluorescence microscopy. Samples were taken 48 h after inoculation of maize plants.

Insets in (B) and (C) give an enlarged view of the clamp cell shown. The distribution of the nuclei with respect to the clamp cell is given in the illustrations at the right. For a detailed description, see text. Bars = 5 μm in (A) and (D) and 10 μm in all other panels.