Figure 6.
Clp1 Does Not Act as a Transcriptional Regulator by Itself.
(A) and (B) Strain UVO151 (a1Δb crg1P:clp1) is deleted for the entire b-locus and harbors an arabinose-inducible version of the clp1 gene integrated into the ip locus as described (Brachmann et al., 2001). The expression of clp1 was induced by 2 and 5 h of growth in arabinose-containing medium. As a control, strain JB1 (a1Δb) was grown under identical conditions.
(A) RNA gel blot analysis of clp1 induction; 5 μg of total RNA were loaded per lane. As a loading control, the filter was stained with methylene blue to visualize blotted rRNA. A 32P-labeled, 0.7-kb fragment of clp1 was used as probe.
(B) MA plot (Yang et al., 2002) illustrating Affymetrix GeneChip results. Results represent two independent experiments. Expression profiles of UVO151 (experiment [E]) and JB1 (baseline [B]) were compared. Ratios of expression values were plotted versus signal intensities. Genes that were not consistently detected in either JB1 or UVO151 are not shown. Data analysis revealed no significant changes in gene expression, with the exception of the 390-fold-induced expression level of clp1 (arrow). The entire data set can be viewed at http://www.ncbi.nlm.nih.gov/projects/geo/ (accession number GSE4689).