Figure 4.

Reduction of E2FC Level Affects Plant Development and Reduces Ploidy Level.

(A) E2FC expression level was analyzed in several independent plant lines (1 to 5) that express an E2FC RNAi (e2fc-R) by semiquantitative RT-PCR of mRNA extracted from 6-d-old seedlings. The ACTIN2 (ACT2) gene was amplified as a control.

(B) E2FC protein levels were analyzed by immunoblotting in the wild type and two lines of e2fc-R (lines 1 and 4) with reduced E2FC mRNA levels. LC, loading control, corresponding to the same blot stained with Coomassie blue.

(C) Phenotypes of 4-d-old wild-type and e2fc-R seedlings.

(D) Phenotypes of 24-d-old wild-type and e2fc-R plants.

(E) Scanning electron micrographs showing epidermal cells of the fourth leaf of 24-d-old wild-type and e2fc-R plants. Bars = 200 μm.

(F) Frequency distribution of epidermal cells with different areas. Measurements were taken using the fourth rosette leaf of 24-d-old wild-type and e2fc-R plants.

(G) Ploidy distribution of fourth rosette leaf nuclei of 24-d-old wild-type and e2fc-R plants.