Figure 3.

Comparison of the Effect of SYP21 Overexpression with That of the Drug Wortmannin.

(A) Individual cellular and extracellular α-amylase activities from experiments shown in Figure 2A (given in ΔOD per milliliter of protoplast suspension and per minute). Mock-electroporated sample was used as blank for all α-amylase assays. Data are from five independent protoplast transfections ± se.

(B) Protoplasts were transfected with a constant amount (10 μg) of plasmid encoding the vacuolar cargo Amy-spo, distributed in equal portions, and then incubated with different concentrations of wortmannin (indicated in micromolar below each lane) for 24 h. Cells and media from five independent protoplast transfections were harvested, and α-amylase activity was measured in each fraction. All other annotations are as in (A).