Figure 8.

Effects of CD and Ory on the Organization of the Central Cytoplasmic Compartment in B. sinuspersici Chlorenchyma Cells.

(A), (D), and (G) Autofluorescence of chloroplasts of chlorenchyma cells incubated over a 2-h period in stabilizing buffer (A) and in the same buffer containing 100 μM CD (D) or 30 μM Ory (G).

(B) and (C) Control chlorenchyma cells were fixed and probed with anti-actin (B) or anti-tubulin (C) antiserum to test the effects of the drugs on the two cytoskeletal elements.

(E) and (F) CD-treated cells probed with actin and tubulin antibodies showing complete disruption of actin filaments (E) with intact microtubules (F).

(H) and (I) Ory-treated chlorenchyma cells with dispersed CCC. The actin filaments remain intact (H), whereas microtubules are completely disrupted (I).

These are representative results from at least three separate experiments. Bars = 10 μm.