Fig. 3. Sedimentation coefficient distribution analysis of apo-, ATPγS-bound and AMP-PNP-bound HP0525. (A) Sedimentation velocity data of 5 µM HP0525 centrifuged at 40 000 r.p.m. Lines represent linear fits of the continuous c(s) conformational change model when constraining the molecular mass to 225 kDa, as a model for the sedimentation coefficient distribution of hexameric HP0525. Different colors are used to distinguish between successive data collection times. (B) Residuals from the linear fits described in (A). (C) Sedimentation coefficient distributions c(s) from the conformational change model.