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. 2003 Jun 1;111(11):1651–1664. doi: 10.1172/JCI17261

Figure 2.

Figure 2

The transcriptional regulation of SRE and AP-1 by estrogens requires the Src/Shc/ERK and JNK signaling pathways, respectively. HeLa cells were transfected with the SRE (a) or the AP-1 (b) reporter constructs used in Figure 1, together with the full-length ERα. Aliquots of these cells were then also transfected with either WT or dn mutants of MEK, Src, or Shc (a). Src K– indicates a mutant lacking the kinase activity of Src; in dn Shc mutants the primary sites of phosphorylation have been substituted by phenylalanine: Y239F/Y240F/Y317F (Shc FFF), Y317F (Shc YYF), or Y239F/Y240F (Shc FFY). (b) HeLa cells were transfected with the AP-1 reporter, ERα, and WT JNK1, dn JNK1, dn MEK, or dn AP-1. One hundred percent indicates the activity in vehicle-treated cells. Bars indicate means ± SD of triplicate determinations; *P < 0.05 versus vehicle by ANOVA.