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Expression of p21Cip1 and p53 in human lung cancer cells after depsipeptide treatment. Cells were treated with depsipeptide (0.0125 to 0.2 μM for 6 h), washed with cold PBS, and incubated at 37°C for 24 h. Protein was harvested, and Western immunoblot analysis was performed to detect the expression of p21Cip1 (A, C, and D) or p53 (B and E) in response to depsipeptide treatment. H1299 cells have no endogenous p53.
